利用一组新的单克隆抗体对新城疫病毒核衣壳蛋白进行抗原特性分析。

Antigenic characterization of the nucleocapsid protein of Newcastle disease virus by means of a new panel of monoclonal antibodies.

作者信息

Panshin A, Shihmanter E, Weisman Y, Orvell C, Kydyrmanov A, Asanov N, Daulbaeva K, Sayatov M, Lipkind M

机构信息

Division of Avian Diseases, Kimron Veterinary Institute, Beit Dagan, Israel.

出版信息

Comp Immunol Microbiol Infect Dis. 2000 Jul;23(3):209-20. doi: 10.1016/s0147-9571(99)00077-6.

DOI:10.1016/s0147-9571(99)00077-6

PMID:10855666

Abstract

Nine monoclonal antibodies (MAB) against nucleocapsid protein (NP) of Newcastle disease virus (NDV) have been prepared and characterized. All the MABs were classified into three groups by means of the competitive binding assay. At least three antigenic sites were delineated on the NP. The 1st site includes two closely located epitopes; the 2nd site includes two related and two distinct epitopes; the 3rd site includes two closely related and one distinct epitopes.

摘要

已制备并鉴定了九种针对新城疫病毒（NDV）核衣壳蛋白（NP）的单克隆抗体（MAB）。通过竞争结合试验将所有单克隆抗体分为三组。在核衣壳蛋白上划定了至少三个抗原位点。第1个位点包括两个紧密相邻的表位；第2个位点包括两个相关和两个不同的表位；第3个位点包括两个密切相关和一个不同的表位。