Anderson L J, Hierholzer J C, Tsou C, Hendry R M, Fernie B F, Stone Y, McIntosh K
J Infect Dis. 1985 Apr;151(4):626-33. doi: 10.1093/infdis/151.4.626.
To study the antigenic characteristics of respiratory syncytial virus (RSV), we developed and evaluated monoclonal antibodies (MAbs) to three strains of RSV: 11 to Long, 4 to 18537, and 9 to A2. Six of these MAbs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion protein. By the pattern of the reactions of these MAbs to 16 strains of RSV in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay, we were able to distinguish three subgroups. With a panel of 10 of these 24 MAbs, we tested 26 strains isolated between 1979 and 1982 in Boston and found that 22 belonged to group 1, 4 to group 2, and none to group 3. The pattern of the reactions of the MAbs against representative strains from the three groups identified nine epitopes by indirect immunofluorescence assay: three of each on the nucleoprotein, the large glycoprotein, and the fusion protein. These results, along with those of previous studies, suggest that groups 1 and 3 are antigenically similar and group 2 is antigenically more distinct.
为研究呼吸道合胞病毒（RSV）的抗原特性，我们制备并评估了针对三株RSV的单克隆抗体（MAb）：针对Long株的11号抗体、针对18537株的4号抗体以及针对A2株的9号抗体。这些单克隆抗体中，6株能免疫沉淀核蛋白，6株能免疫沉淀大糖蛋白，11株能免疫沉淀融合蛋白。通过这些单克隆抗体在间接免疫荧光试验或酶联免疫吸附试验中与16株RSV的反应模式，我们能够区分出三个亚组。用这24株单克隆抗体中的10株组成一组，我们检测了1979年至1982年期间在波士顿分离出的26株病毒，发现其中22株属于第1组，4株属于第2组，无属于第3组的。这些单克隆抗体与来自三组的代表性毒株的反应模式通过间接免疫荧光试验确定了9个表位：核蛋白、大糖蛋白和融合蛋白上各有3个。这些结果以及先前研究的结果表明，第1组和第3组在抗原性上相似，而第2组在抗原性上更具独特性。
