Orvell C, Rydbeck R, Löve A
J Gen Virol. 1986 Sep;67 ( Pt 9):1929-39. doi: 10.1099/0022-1317-67-9-1929.
The immunological relationships between mumps virus and parainfluenza viruses were investigated with 74, 78 and 80 previously developed monoclonal antibodies directed against five major structural proteins of mumps virus, Sendai virus (a murine parainfluenza type 1 virus) and parainfluenza type 3 virus. These monoclonal antibodies were reacted with the three viruses, with parainfluenza type 2 virus and with Newcastle disease virus (NDV) in ELISA and immunofluorescence (IF) tests. In addition, immunoprecipitation tests with [35S]methionine-labelled extracellular virions were carried out with cross-reacting monoclonal antibodies. None of all 232 monoclonal antibodies against the three viruses cross-reacted with either parainfluenza type 2 virus or NDV in ELISA and IF tests. In the collection of 74 mumps virus monoclonal antibodies, three directed against the nucleocapsid (NP) protein, polymerase protein, and fusion protein cross-reacted with Sendai virus. Two Sendai virus monoclonal antibodies directed against two different epitopes of the haemagglutinin-neuraminidase (HN) protein cross-reacted with parainfluenza type 3 virus. Six other Sendai virus monoclonal antibodies directed against four different epitopes of the HN protein and one directed against the NP protein cross-reacted with mumps virus. Eight out of 80 monoclonal antibodies directed against parainfluenza type 3 virus cross-reacted with Sendai virus. One was directed against the HN protein, four were directed against a minimum of two epitopes of the matrix protein and three were directed against three different epitopes of the NP protein. The different cross-reactions found show that Sendai virus is antigenically related to both mumps virus and parainfluenza type 3 virus. In contrast, no antigenic relationship could be demonstrated between mumps virus and parainfluenza type 3 virus.
利用74种、78种和80种先前研制的针对腮腺炎病毒、仙台病毒（一种鼠源1型副流感病毒）和3型副流感病毒五种主要结构蛋白的单克隆抗体，对腮腺炎病毒和副流感病毒之间的免疫关系进行了研究。这些单克隆抗体在酶联免疫吸附测定（ELISA）和免疫荧光（IF）试验中与这三种病毒、2型副流感病毒以及新城疫病毒（NDV）发生反应。此外，还使用交叉反应性单克隆抗体对用[35S]甲硫氨酸标记的细胞外病毒粒子进行了免疫沉淀试验。在针对这三种病毒的所有232种单克隆抗体中，没有一种在ELISA和IF试验中与2型副流感病毒或NDV发生交叉反应。在74种腮腺炎病毒单克隆抗体中，有三种针对核衣壳（NP）蛋白、聚合酶蛋白和融合蛋白的抗体与仙台病毒发生交叉反应。两种针对血凝素神经氨酸酶（HN）蛋白两个不同表位的仙台病毒单克隆抗体与3型副流感病毒发生交叉反应。另外六种针对HN蛋白四个不同表位的仙台病毒单克隆抗体和一种针对NP蛋白的抗体与腮腺炎病毒发生交叉反应。在针对3型副流感病毒的80种单克隆抗体中，有八种与仙台病毒发生交叉反应。一种针对HN蛋白，四种针对基质蛋白至少两个表位，三种针对NP蛋白的三个不同表位。所发现的不同交叉反应表明，仙台病毒在抗原性上与腮腺炎病毒和3型副流感病毒均相关。相比之下，未证明腮腺炎病毒和3型副流感病毒之间存在抗原关系。
