通过实时聚合酶链反应快速检测临床样本中的肺炎支原体

Rapid detection of Mycoplasma pneumoniae in clinical samples by real-time PCR.

作者信息

Hardegger D, Nadal D, Bossart W, Altwegg M, Dutly F

机构信息

Department of Medical Microbiology, University of Zürich, Switzerland.

出版信息

J Microbiol Methods. 2000 Jun;41(1):45-51. doi: 10.1016/s0167-7012(00)00135-4.

DOI:10.1016/s0167-7012(00)00135-4

PMID:10856776

Abstract

M. pneumoniae is a common causative agent of community-acquired pneumonia in children. The diagnosis of such infections is usually based on serology using complement fixation or, more recently, enzyme-immuno assays. PCR has been shown to be a promising alternative. We have evaluated a real-time PCR assay targeting the P1 adhesion protein gene and compared it to a conventional semi-nested PCR assay with the 16S rDNA as target. Comparison of 147 specimens from 48 patients showed an overall agreement of 97.4%. Real-time PCR proved to be of equal value on clinical specimens as conventional PCR regarding sensitivity and specificity, but is clearly advantageous regarding speed, handling and number of samples that can be analyzed per run.

摘要

肺炎支原体是儿童社区获得性肺炎的常见病原体。此类感染的诊断通常基于使用补体结合试验的血清学检测，或者更近一些，基于酶免疫测定。聚合酶链反应（PCR）已被证明是一种很有前景的替代方法。我们评估了一种针对P1黏附蛋白基因的实时PCR检测方法，并将其与以16S核糖体DNA为靶标的传统半巢式PCR检测方法进行比较。对48例患者的147份标本进行比较，结果显示总体一致性为97.4%。在临床标本上，实时PCR在敏感性和特异性方面与传统PCR具有同等价值，但在速度、操作以及每次运行可分析的样本数量方面明显更具优势。