Jewett M A, Gupta S, Hansen J A, Cunningham-Rundles S, Siegal F P, Good R A, Dupont B
Clin Exp Immunol. 1976 Sep;25(3):449-54.
The responses of fresh and frozen lymphocytes to mitogens and antigens have been compared using samples collected on five separate occasions from one normal donor. The day-to-day variation seen with the fresh cells was eliminated by the use of frozen cells. Thawed cells from one donor collected on one occasion but studied on five separate occasions and compared to fresh cells on the same days, showed fluctuations from day to day as well, confirming that the day-to-day variation seen is due to technical and not biological phenomena. Cryopreserved cells showed a decrease in responses to specific microbial antigens, a slight shift in the PHA dose-response curve, but no significant difference in responses to Con A or PWM. The relative proportion of lymphocyte subpopulations changed with freezing and thawing. The proportion of T cells increased slightly and the proportion of B cells decreased.
使用从一名正常供体在五个不同时间点采集的样本，比较了新鲜淋巴细胞和冷冻淋巴细胞对有丝分裂原和抗原的反应。使用冷冻细胞消除了新鲜细胞中出现的每日变化。从一名供体在某一时刻采集但在五个不同时间点进行研究并与同一天的新鲜细胞进行比较的解冻细胞，也显示出每日波动，证实所观察到的每日变化是由于技术而非生物学现象。冷冻保存的细胞对特定微生物抗原的反应降低，PHA剂量反应曲线略有偏移，但对Con A或PWM的反应无显著差异。淋巴细胞亚群的相对比例随冷冻和解冻而变化。T细胞比例略有增加，B细胞比例下降。
