Jewett M A
Natl Cancer Inst Monogr. 1978 Dec(49):89-92.
The responses of fresh and frozen lymphocytes to mitogens and antigens have been compared with the use of samples collected on five occasions from 1 normal donor. The day-to-day variation seen with the fresh cells was eliminated by the use of frozen cells. Thawed cells from 1 donor, collected once but studied on five occasions and compared with fresh cells on the same days, fluctuated from day to day as well, confirming that the day-to-day variation seen is due to technical and not biologic phenomena. Cryopreserved cells showed a decrease in responses to specific microbial antigens, a slight shift in the PHA dose-response curve, but no significant difference in responses to Con A or PWM. The relative proportion of lymphocyte subpopulations changed with freezing and thawing. The proportion of T- and B-cells increased and decreased, respectively.
使用从1名正常供体在五个不同时间点采集的样本，比较了新鲜淋巴细胞和冷冻淋巴细胞对有丝分裂原和抗原的反应。使用冷冻细胞消除了新鲜细胞中观察到的每日变化。来自1名供体的解冻细胞，虽然只采集了一次，但在五个不同时间点进行研究，并与同一天的新鲜细胞进行比较，结果显示每天也有波动，这证实观察到的每日变化是由于技术因素而非生物学现象所致。冷冻保存的细胞对特定微生物抗原的反应有所降低，PHA剂量反应曲线略有偏移，但对刀豆蛋白A或美洲商陆有丝分裂原的反应无显著差异。淋巴细胞亚群的相对比例随冻融而变化。T细胞和B细胞的比例分别增加和减少。
