冷冻保存的淋巴细胞在混合淋巴细胞反应中以及与植物血凝素和特异性抗原一起培养时的体外转化。
The in vitro transformation of frozen-stored lymphocytes in the mixed lymphocyte reaction and in culture with phytohemagglutinin and specific antigens.
作者信息
Mangi R J, Mardiney M R
出版信息
J Exp Med. 1970 Sep 1;132(3):401-16. doi: 10.1084/jem.132.3.401.
Abstract
We have investigated the in vitro transformation of lymphocytes that have been frozen and stored at low temperatures. Cells frozen at different times and stored for various times do transform in a reproducible manner when placed in culture with PHA or as one population of the two-way or the one-way MLR. When frozen-stored lymphocytes are cultured with specific antigen or as both partners in the MLR the response is minimal. The freezing process destroys neutrophils, and the remaining population transforms in a manner similar to cultures of purified lymphocytes. Practical aspects of the technique are discussed and we have suggested possible practical applications for future investigation.
摘要
我们研究了在低温下冷冻保存的淋巴细胞的体外转化。在不同时间冷冻并保存不同时长的细胞，当与PHA一起培养时，或作为双向或单向混合淋巴细胞反应中的一个群体时，确实会以可重复的方式发生转化。当冷冻保存的淋巴细胞与特异性抗原一起培养时，或作为混合淋巴细胞反应中的双方时，反应极小。冷冻过程会破坏中性粒细胞，剩余的细胞群体转化方式与纯化淋巴细胞培养物相似。讨论了该技术的实际应用方面，并且我们提出了未来研究可能的实际应用。
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