Means G D, Toy D Y, Baum P R, Derry J M
Immunex Corporation, Seattle, Washington 98101-2936, USA.
Genomics. 2000 May 1;65(3):213-23. doi: 10.1006/geno.2000.6173.
A physical clone contig has been constructed, spanning 2 Mb on the proximal mouse X chromosome containing the mouse scurfy (sf) and tattered (Td) mutations. Extensive transcript mapping in this interval has identified 37 potential transcription units, including a number of novel genes, and 4 pseudogenes. These genes have been ordered by STS content and restriction mapping. Comparison of the transcript map to the corresponding region in human Xp11.23-p11.22 shows extensive homology, with complete conservation of gene order for loci in common between the two maps. Further, using a novel method to identify simple sequence length polymorphisms, we have developed a number of genetic markers, which has enabled the region containing the sf mutation to be narrowed to <300 kb. This contig has already allowed the cloning of the Td gene using a candidate gene approach and now serves as a starting point for the cloning of the sf mutation.
已经构建了一个物理克隆重叠群，它跨越小鼠X染色体近端2 Mb的区域，该区域包含小鼠的鳞癣（sf）和破烂（Td）突变。在这个区间进行的广泛转录本图谱分析已鉴定出37个潜在的转录单位，包括一些新基因和4个假基因。这些基因已根据STS含量和限制性图谱进行了排序。将转录本图谱与人类Xp11.23 - p11.22的相应区域进行比较，发现有广泛的同源性，两张图谱中共有位点的基因顺序完全保守。此外，使用一种鉴定简单序列长度多态性的新方法，我们开发了一些遗传标记，这使得包含sf突变的区域缩小到<300 kb。这个重叠群已经通过候选基因方法实现了Td基因的克隆，现在作为克隆sf突变的起点。
