Besse A, Trimoreau F, Praloran V, Denizot Y
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Cytokine. 2000 May;12(5):522-5. doi: 10.1006/cyto.1999.0580.
We have investigated the effect of growth factors, inflammatory and anti-inflammatory cytokines on the macrophage colony-stimulating factor (M-CSF) secretion by cultured human bone marrow stromal cells. Their production of M-CSF cultured in serum-free medium is enhanced in a time-dependent manner in response to tumour necrosis factor (TNF-)alpha and interleukin (IL-)4 but not to IL-1, IL-3, IL-6, IL-7, IL-10, SCF, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, bFGF and transforming growth factor (TGF-)beta. The co-addition of IL-4 and TNF-alpha has a greater than additive effect on the secretion of M-CSF suggesting that they act synergistically. The anti-inflammatory molecules IL-10 and TGF-beta have no effect on the TNF-alpha-induced M-CSF synthesis by marrow stromal cells. In conclusion TNF-alpha and IL-4 are potent stimulators of the M-CSF synthesis by human bone marrow stromal cells, a result of importance regarding the role of M-CSF in the proliferation/differentiation of mononuclear-phagocytic cells and the role of marrow stromal cells as regulators of marrow haematopoiesis.
我们研究了生长因子、炎性和抗炎细胞因子对培养的人骨髓基质细胞分泌巨噬细胞集落刺激因子（M-CSF）的影响。在无血清培养基中培养时，它们对M-CSF的产生会因肿瘤坏死因子（TNF-）α和白细胞介素（IL-）4而呈时间依赖性增强，但对IL-1、IL-3、IL-6、IL-7、IL-10、干细胞因子（SCF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、粒细胞集落刺激因子（G-CSF）、碱性成纤维细胞生长因子（bFGF）和转化生长因子（TGF-）β无反应。IL-4和TNF-α共同添加对M-CSF的分泌具有大于相加的作用，表明它们协同发挥作用。抗炎分子IL-10和TGF-β对骨髓基质细胞由TNF-α诱导的M-CSF合成无影响。总之，TNF-α和IL-4是人类骨髓基质细胞合成M-CSF的有效刺激物，这一结果对于M-CSF在单核吞噬细胞增殖/分化中的作用以及骨髓基质细胞作为骨髓造血调节因子的作用具有重要意义。
