Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
Int J Biol Sci. 2011 Apr 1;7(3):364-75. doi: 10.7150/ijbs.7.364.
Increasing evidence shows that some cells from peripheral blood fibroblast-like mononuclear cells have the capacity to differentiate into mesenchymal lineages. However, the insufficiency of these cells in the circulation challenges the cell isolation and subsequently limits the clinical application of these cells. In the present study, the peripheral blood mononuclear cells (pbMNCs) were isolated from wound animals and treated with the supernatant of bone marrow mesenchymal stromal cells (bmMSCs). Results showed these pbMNCs were fibroblast-like, had stromal morphology, were negative for CD34 and CD45, but positive for Vimentin and Collagen I, and had the multipotency to differentiate into adipocytes and osteoblasts. We named these induced peripheral blood-derived mesenchymal stromal cells (ipbMSCs). Skin grafts in combination with ipbMSCs and collagen I were applied for wound healing, and results revealed ipbMSC exhibited similar potency and effectiveness in the promotion of wound healing to the bmMSCs. Hereafter, we speculate that the mixture of growth factors and chemokines secreted by bmMSCs may play an important roles in the induction of the proliferation and mesenchymal differentiation of mononuclear cells. Our results are clinically relevant because it provide a new method for the acquisition of MSCs which can be used as a candidate for the wound repair.
越来越多的证据表明，外周血成纤维样单核细胞中的一些细胞具有向间充质谱系分化的能力。然而，这些细胞在循环中的不足挑战了细胞的分离，进而限制了这些细胞的临床应用。在本研究中，从创伤动物中分离外周血单个核细胞（pbMNCs），并用骨髓间充质基质细胞（bmMSCs）的上清液处理。结果表明这些 pbMNCs 呈成纤维样，具有基质形态，CD34 和 CD45 阴性，但波形蛋白和胶原 I 阳性，具有向脂肪细胞和成骨细胞分化的多能性。我们将这些诱导的外周血源性间充质基质细胞（ipbMSCs）命名为这些细胞。将皮肤移植物与 ipbMSCs 和胶原 I 联合用于伤口愈合，结果表明 ipbMSC 在促进伤口愈合方面与 bmMSCs 具有相似的功效。此后，我们推测 bmMSCs 分泌的生长因子和趋化因子混合物可能在单核细胞的增殖和间充质分化诱导中发挥重要作用。我们的研究结果具有临床相关性，因为它为获得可作为伤口修复候选物的 MSC 提供了一种新方法。
