Denizot Y, Raher S, Trimoreau F, Praloran V, Godard A
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Cytokine. 2000 May;12(5):499-502. doi: 10.1006/cyto.1999.0578.
This study investigates the production of interleukin (IL-)1beta by cultured human bone marrow stromal cells. RT-PCR experiments indicate that two-thirds of cultures constitutively express IL-1beta mRNA transcripts. Their cell-associated IL-1beta levels are elevated after stimulation with tumour necrosis factor (TNF-)alpha but not with cytokines such as IL-1alpha, IL-3, IL-4, IL-6, IL-7, IL-10, SCF, G-CSF, M-CSF and TGF-beta or lipid mediators such as PGE2, LTB4, LXA4, LXB4, 12-HETE, 15-HETE and PAF. Addition of IL-4, but not IL-10 or TGF-beta, reduces the TNF-alpha-induced cell-associated IL-1beta. IL-1beta is never detected in bone marrow stromal cell supernatants whatever the stimulant added. In conclusion the pro-inflammatory molecule TNF-alpha stimulates bone marrow stromal cell-associated IL-1beta levels while the anti-inflammatory cytokine IL-4 reduces the TNF-alpha-induced effect. These results strengthen the key regulatory role of IL-4 on the production of haematopoietic cytokines by human bone marrow stromal cells.
本研究调查了培养的人骨髓基质细胞白细胞介素（IL-）-1β的产生。逆转录聚合酶链反应（RT-PCR）实验表明，三分之二的培养物组成性表达IL-1β信使核糖核酸（mRNA）转录本。在用肿瘤坏死因子（TNF-）-α刺激后，它们的细胞相关IL-1β水平升高，但用诸如IL-1α、IL-3、IL-4、IL-6、IL-7、IL-10、干细胞因子（SCF）、粒细胞集落刺激因子（G-CSF）、巨噬细胞集落刺激因子（M-CSF）和转化生长因子（TGF）-β等细胞因子或诸如前列腺素E2（PGE2）、白三烯B4（LTB4）、脂氧素A4（LXA4）、脂氧素B4（LXB4）、12-羟基二十碳四烯酸（12-HETE）、15-羟基二十碳四烯酸（15-HETE）和血小板活化因子（PAF）等脂质介质刺激后则不升高。添加IL-4可降低TNF-α诱导的细胞相关IL-1β，但添加IL-10或TGF-β则不能。无论添加何种刺激物，在骨髓基质细胞上清液中均未检测到IL-1β。总之，促炎分子TNF-α刺激骨髓基质细胞相关的IL-1β水平，而抗炎细胞因子IL-4则降低TNF-α诱导的效应。这些结果强化了IL-4对人骨髓基质细胞造血细胞因子产生的关键调节作用。
