Nakano M, Morimoto Y, Tajima S, Kosaka N
Kampo and Healthcare Research Laboratories, Kanebo, Ltd., Osaka, Japan.
Yakugaku Zasshi. 2000 Jun;120(6):583-6. doi: 10.1248/yakushi1947.120.6_583.
To determine l-ephedrine (E) and d-pseudoephedrine (PE) concentrations in human plasma simultaneously, we used a selected-ion monitoring method with gas chromatography-mass spectrometry (GC-MS) using deuterium-labeled E and PE as internal standards. The E and PE in human plasma were extracted with hexane-ethylacetate (9/1) under alkaline conditions and were easily converted into their heptafluorobutyryl derivatives by treating with heptafluorobutyrylimidazole. The calibration curves of E and PE showed a good linearity in the range from 0.82 to 81.9 ng/ml for E, and from 0.41 to 41.0 ng/ml for PE, respectively. The limit of detection was 0.82 ng/ml for E and 0.41 ng/ml for PE in human plasma, respectively.
为了同时测定人血浆中l-麻黄碱（E）和d-伪麻黄碱（PE）的浓度，我们使用了气相色谱-质谱联用（GC-MS）的选择离子监测方法，以氘标记的E和PE作为内标。人血浆中的E和PE在碱性条件下用己烷-乙酸乙酯（9/1）萃取，并用七氟丁酰咪唑处理后很容易转化为它们的七氟丁酰衍生物。E和PE的校准曲线分别在0.82至81.9 ng/ml（E）和0.41至41.0 ng/ml（PE）范围内显示出良好的线性。人血浆中E的检测限分别为0.82 ng/ml，PE的检测限为0.41 ng/ml。
