Yang L L, Tu X D
Nanjing General Hospital of PLA.
Yao Xue Xue Bao. 1993;28(9):709-13.
A specific and sensitive method has been established for the analysis of pseudoephedrine (PE) in human plasma by gas chromatography with a mass selective detector (GC-MSD). PE and ephedrine (E, internal standard) reacted with trifluoroacetic anhydride (TFAA) and the derivatives were separated by a HP-5 capillary column. PE-TFA and E-TFA were quantitatively measured by selected ion monitoring (m/z = 154). The detection limits for E and PE were 1 ng/ml plasma. The recoveries were about 99%.
已建立一种采用气相色谱-质量选择检测器（GC-MSD）分析人血浆中伪麻黄碱（PE）的特异且灵敏的方法。PE和麻黄碱（E，内标）与三氟乙酸酐（TFAA）反应，其衍生物通过HP-5毛细管柱进行分离。通过选择离子监测（m/z = 154）对PE-TFA和E-TFA进行定量测定。E和PE的检测限为1 ng/ml血浆。回收率约为99%。
