Atabekov J G, Rodionova N P, Karpova O V, Kozlovsky S V, Poljakov V Y
Department of Virology, Moscow State University, Russia.
Virology. 2000 Jun 5;271(2):259-63. doi: 10.1006/viro.2000.0319.
Plant virus-encoded movement protein(s) (MP), and for many viruses the coat protein (CP), is required to mediate viral spread between plant cells via plasmodesmata (PD). Most probably, the genomic RNA of potexviruses moves through PD as assembled virions and/or as ribonucleoprotein complexes containing the CP and 25-kDa MP. Here we report that encapsidated potato virus X (PVX) virion RNA, which is nontranslatable in a cell-free protein synthesizing system, can be converted into a fully translatable form after interaction of intact PVX particles with the PVX 25-kDa MP. The 25-kDa MP molecules bind selectively to only one extremity of the viral particle (that presumably contains the 5' end of the genomic RNA). The process of complex formation is ATP-independent; i.e., the ATPase activity of the 25-kDa MP is not involved in the binding of the MP to PVX virion.
植物病毒编码的运动蛋白（MP），对于许多病毒而言还有外壳蛋白（CP），是介导病毒通过胞间连丝（PD）在植物细胞间传播所必需的。很可能，马铃薯X病毒属病毒的基因组RNA以组装好的病毒粒子和/或包含CP和25 kDa MP的核糖核蛋白复合体的形式通过PD移动。在此我们报告，在无细胞蛋白质合成系统中不可翻译的衣壳化马铃薯X病毒（PVX）病毒粒子RNA，在完整的PVX颗粒与PVX 25 kDa MP相互作用后可转化为完全可翻译的形式。25 kDa MP分子仅选择性地结合到病毒粒子的一个末端（推测该末端包含基因组RNA的5'端）。复合物形成过程不依赖ATP；也就是说，25 kDa MP的ATP酶活性不参与MP与PVX病毒粒子的结合。
