Arkhipenko M V, Nikitin N A, Donchenko E K, Karpova O V, Atabekov J G
Biology Department, Lomonosov Moscow State University, 1 bld. 12 Leninskie gory, Moscow, 119234 , Russia.
Acta Naturae. 2017 Oct-Dec;9(4):52-57.
We had shown the genomic RNA of potexviruses potato virus X and the alternanthera mosaic virus to be inaccessible to ribosomes while in intact virion form, but the RNAs can be translationally activated following the binding of movement protein 1 (MP1) to virus particles. Here, we present the results of the follow-up study targeting two more potexvirus species - the Narcissus mosaic virus and the Potato aucuba mosaic virus. We found encapsidated potexviral RNA to share common translational features and the MP1 to be potent over homological virions of its "own" species and over heterological virions of other species, as well exhibiting selective specificity. Reciprocal cross-activation is observed among viral species phylogenetically either close or distant. There is direct evidence that MP1 binding to the end of the virion is necessary, but not sufficient, for translational activation of encapsidated RNA.
我们已表明，马铃薯X病毒和莲子草花叶病毒这两种马铃薯Y病毒属病毒的基因组RNA在完整病毒粒子形式下无法被核糖体识别，但在运动蛋白1（MP1）与病毒粒子结合后，这些RNA可被翻译激活。在此，我们展示了针对另外两种马铃薯Y病毒属病毒——水仙花叶病毒和马铃薯奥古巴花叶病毒的后续研究结果。我们发现，被包裹的马铃薯Y病毒属病毒RNA具有共同的翻译特征，且MP1对其“自身”物种的同源病毒粒子以及其他物种的异源病毒粒子均具有强大作用，同时还表现出选择性特异性。在系统发育上相近或较远的病毒物种之间均观察到了相互交叉激活现象。有直接证据表明，MP1与病毒粒子末端的结合对于被包裹RNA的翻译激活是必要的，但并不充分。
