Best-Belpomme M, Richard-Foy H, Secco-Millet C, Baulieu E E
Biochimie. 1976;58(7):863-9. doi: 10.1016/s0300-9084(76)80317-3.
Estradiol-PAB-cellulose, an easily prepared adsorbent, has been proposed to purify the uterine estradiol receptor according to the principle of biospecific affinity chromatography. It apparently removes all hormone binding sites when cytosol preparations are incubated with it. A systematic study of this adsorbent was undertaken, including the synthesis and testing of the radioactive material. Two main results were obtained: 1) Estradiol-PAB-cellulose is heavily contaminated with free ligand and releases it during the normal chromatographic conditions. 2) Estradiol spacer derivatives (hydroxyethylphenyl-diazo (2 or 4)-estradiol) have a very low affinity for the receptor (Ki = 10 muM). The conclusion is that estradiol-PAB-cellulose is unsuitable for affinity chromatography of estradiol receptor.
雌二醇-对氨基苯甲酰纤维素是一种易于制备的吸附剂，根据生物特异性亲和色谱原理，已有人提出用它来纯化子宫雌二醇受体。当用它孵育胞质溶胶制剂时，它显然会去除所有激素结合位点。对这种吸附剂进行了系统研究，包括放射性物质的合成和测试。得到了两个主要结果：1）雌二醇-对氨基苯甲酰纤维素被游离配体严重污染，并在正常色谱条件下释放出来。2）雌二醇间隔基衍生物（羟乙基苯基重氮（2或4）-雌二醇）对受体的亲和力非常低（解离常数Ki = 10微摩尔）。结论是雌二醇-对氨基苯甲酰纤维素不适用于雌二醇受体的亲和色谱分析。
