Kushnirov V V
Institute of Experimental Cardiology, Cardiology Research Centre, Moscow, Russia.
Yeast. 2000 Jun 30;16(9):857-60. doi: 10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B.
The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates.
目前用于从酵母中提取蛋白质的方法要么费力，要么可靠性不足。在此，我报告一种用于电泳分析的蛋白质提取方法，该方法既简便又可靠。在这种方法中，酵母细胞经过温和的碱处理，然后在标准电泳上样缓冲液中煮沸。该方法针对酿酒酵母的不同菌株以及多形汉逊酵母DL-1进行了测试。它几乎能完全提取蛋白质，与菌株、生长条件和蛋白质分子量无关，并且允许使用在琼脂平板上生长的少量酵母细胞进行操作。
