血小板活化因子对小鼠精子获能和顶体反应的影响。
Effects of platelet activating factor on capacitation and acrosome reaction in mouse spermatozoa.
作者信息
Huo L J, Yang Z M
机构信息
Department of Biotechnology, Northeast Agricultural University, Harbin, China.
出版信息
Mol Reprod Dev. 2000 Jul;56(3):436-40. doi: 10.1002/1098-2795(200007)56:3<436::AID-MRD14>3.0.CO;2-L.
Platelet activating factor (PAF) plays an important role in mammalian reproduction. The aim of this study was to investigate the effects of PAF on capacitation and acrosome reaction of mouse spermatozoa by chlortetracycline (CTC) fluorescence assay and coomassie blue staining. The percentage of capacitated mouse spermatozoa was increased (P < 0.05) by incubation with 50 ng/ml PAF for 20-120 min. The peak response occurred between 80 to 100 min of exposure to PAF. In contrast, the effects of PAF on acrosome reaction may be not receptor-mediated since lyso-PAF had the same effects. Ionophore A23187 stimulated an increase in acrosome-reacted spermatozoa of PAF-treated spermatozoa, but not of lyso-PAF-treated ones. These results suggest that PAF mainly acts on sperm capacitation.
血小板活化因子（PAF）在哺乳动物生殖过程中发挥着重要作用。本研究旨在通过金霉素（CTC）荧光测定法和考马斯亮蓝染色法，研究PAF对小鼠精子获能和顶体反应的影响。用50 ng/ml PAF孵育20 - 120分钟后，获能小鼠精子的百分比增加（P < 0.05）。在暴露于PAF 80至100分钟之间出现峰值反应。相比之下，PAF对顶体反应的影响可能不是受体介导的，因为溶血PAF具有相同的作用。离子载体A23187刺激了PAF处理的精子顶体反应精子的增加，但对溶血PAF处理的精子没有作用。这些结果表明，PAF主要作用于精子获能。
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