血小板激活因子通过激活人精子细胞中的细胞外信号调节激酶诱导顶体反应。

Platelet-activating factor induces acrosome reaction via the activation of extracellular signal-regulated kinase in human spermatozoa.

机构信息

Clinic of Reproductive Medicine, Jiangmen Central Hospital, Jiangmen, China.

Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.

出版信息

Andrologia. 2020 Jun;52(5):e13565. doi: 10.1111/and.13565. Epub 2020 Mar 18.

DOI:10.1111/and.13565

PMID:32187723

Abstract

Platelet-activating factor (PAF) affects capacitation, acrosome reaction and fertilisation potential of spermatozoa. This study investigated the underlying mechanism(s) through which PAF regulated sperm function. Our data demonstrated that PAF dose-dependently induced, whilst lyso-PAF (PAF precursor) showed no effect on acrosome reaction of capacitated human spermatozoa. Treatment with PAF for 90 min enhanced tyrosine phosphorylation and expression of extracellular signal-regulated protein kinases (ERK) 1 and 2 in human spermatozoa. Moreover, pre-treatment with the ERK inhibitor U0126 significantly and dose-dependently suppressed PAF-induced acrosome reaction. Therefore, PAF may be actively involved in the modulation of sperm acrosome reaction by interacting with ERK. The role of PAF in fertilisation warrants further investigation.

摘要

血小板激活因子（PAF）影响精子的获能、顶体反应和受精潜能。本研究旨在探讨 PAF 调节精子功能的潜在机制。我们的数据表明，PAF 呈浓度依赖性诱导顶体反应，而溶血血小板激活因子（PAF 前体）对获能的人精子顶体反应没有影响。PAF 处理 90 分钟可增强人精子的酪氨酸磷酸化和细胞外信号调节激酶（ERK）1 和 2 的表达。此外，ERK 抑制剂 U0126 的预处理可显著且浓度依赖性地抑制 PAF 诱导的顶体反应。因此，PAF 可能通过与 ERK 相互作用而积极参与精子顶体反应的调节。PAF 在受精中的作用值得进一步研究。