Yoshiki Y, Okubo K, Akiyama Y, Sato K, Kawanari M
Department of Environmental Bioremediation, Graduate School of Agricultural Science, Tohoku University, Japan.
Luminescence. 2000 May-Jun;15(3):183-7. doi: 10.1002/1522-7243(200005/06)15:3<183::AID-BIO584>3.0.CO;2-V.
Photon emission in the hydrogen peroxide/gallic acid/lactoperoxidase system was studied using visible region chemiluminescence. The photon emission intensity showed a pH-dependence curve with a maximum at pH 4.5-5.0. The spectral analysis showed to be at 510 nm and its energy was 61.0 kcal/mol at either pH 4.5 or pH 7.0. In addition, near-infrared spectral analysis at 1270 nm suggested that this photon emission from the hydrogen peroxide/gallic acid/lactoperoxidase system was produced without generation of (1)O(2). The gallic acid/lactoperoxidase system, based on the chemiluminescence system, has superoxide-, hydroxyl radical-scavenging activities and antioxidative activity. These results are strong evidence that the gallic acid/lactoperoxidase system is one of the reactive oxygen scavenging systems.
利用可见区化学发光研究了过氧化氢/没食子酸/乳过氧化物酶体系中的光子发射。光子发射强度呈现出一条pH依赖性曲线，在pH 4.5 - 5.0时达到最大值。光谱分析表明其波长为510 nm，在pH 4.5或pH 7.0时其能量为61.0千卡/摩尔。此外，1270 nm处的近红外光谱分析表明，过氧化氢/没食子酸/乳过氧化物酶体系的这种光子发射是在不产生单线态氧((1)O(2))的情况下产生的。基于化学发光体系的没食子酸/乳过氧化物酶体系具有超氧化物、羟基自由基清除活性和抗氧化活性。这些结果有力地证明了没食子酸/乳过氧化物酶体系是活性氧清除体系之一。
