酶联免疫吸附测定在泌尿生殖系统结核疑似病例诊断中的作用
Role of enzyme linked immunosorbent assay in the diagnosis of suspected cases of genito urinary tuberculosis.
作者信息
Gupta A, Kumar V, Xess A, Sharma H P, Shahi S K
机构信息
Department of Microbiology, Indira Gandhi Institute of Medical Sciences, Patna.
出版信息
Indian J Pathol Microbiol. 1999 Jul;42(3):307-9.
The aim of study was evaluation of the utility of ELISA test using A60 Antigen for rapid diagnosis of Genitourinary Tuberculosis in various age groups. ELISA test based on mycobacterial antigen A60 (Anda biological, France) was used to estimate specific IgG antibodies in the sera of fifty four suspected cases of Genito urinary tuberculosis. (GUT)Sera of 30 montoux negative healthy adults (age/sex matched) were taken as control by detecting IgG anti bodies to A60 antigen. It was concluded from this study that IgM was positive in 87.0% of cases.
本研究的目的是评估使用A60抗原的酶联免疫吸附测定（ELISA）试验在各年龄组中快速诊断泌尿生殖系统结核病的效用。基于分枝杆菌抗原A60（法国安达生物公司）的ELISA试验用于检测54例疑似泌尿生殖系统结核病患者血清中的特异性IgG抗体。通过检测针对A60抗原的IgG抗体，将30名结核菌素试验阴性的健康成年人（年龄/性别匹配）的血清作为对照。本研究得出结论，87.0%的病例中IgM呈阳性。
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