Zigová J
Institute of Biotechnology, Research Centre Juelich, D-52425, Juelich, Germany.
J Biotechnol. 2000 Jun 9;80(1):55-62. doi: 10.1016/s0168-1656(00)00231-5.
A feedback RQ controlled fed-batch process for the recombinant production of a soluble human N-deglycosylated recombinant beta-1, 4-galactosyltransferase (NdrGal-T) with Saccharomyces cerevisiae BT150 was investigated. Several RQ values were tested for optimal production of NdrGal-T. Four times higher volumetric activity was reached at RQ=1.0 (32 U l(-1)) than at all higher RQ values (about 8 U l(-1)). RQ, 1.0 was the best choice for both, biomass and enzyme production. Optimal concentration of glucose in preculture was 25 g l(-1). At higher values slightly more ethanol was produced than at lower values of preculture glucose concentrations, moreover no positive effect on biomass and enzyme production was found. Lower values caused not only decrease of ethanol but also decrease of biomass formation (from 1.69 g h(-1) to 0.81 g h(-1)) and enzyme overall productivity (from 2.2 U h(-1) to 0.63 U h(-1)). Successfully performed cultivation with three precultures predicted scale-up possibility of feedback RQ-controlled NdrGal-T production with S. cerevisiae BT150 from lab to pilot-scale fermentor.
研究了一种采用反馈呼吸商（RQ）控制的补料分批培养过程，用于酿酒酵母BT150重组生产可溶性人N-去糖基化重组β-1,4-半乳糖基转移酶（NdrGal-T）。测试了几个RQ值以实现NdrGal-T的最优生产。在RQ = 1.0（32 U l⁻¹）时达到的体积活性比所有更高RQ值（约8 U l⁻¹）时高四倍。RQ为1.0对生物量和酶的生产都是最佳选择。预培养中葡萄糖的最佳浓度为25 g l⁻¹。葡萄糖浓度较高时，产生的乙醇略多于较低浓度时，此外，对生物量和酶的生产未发现积极影响。较低浓度不仅导致乙醇减少，还导致生物量形成减少（从1.69 g h⁻¹降至0.81 g h⁻¹）和酶的总生产率降低（从2.2 U h⁻¹降至0.63 U h⁻¹）。用三种预培养成功进行的培养预示了采用反馈RQ控制的酿酒酵母BT150生产NdrGal-T从实验室规模扩大到中试规模发酵罐的可能性。
