Sagawa T, Shinohara M
Department of Pharmacology, Osaka Dental University, Japan.
J Osaka Dent Univ. 1999 Apr;33(1):35-41.
It is well known that there are several coagulation factors and fibrinolysis factors, and these inhibitors are also present in blood and tissues. Normally, these factors balance each other. We measured antithrombin III (AT III), an important inhibitor of coagulation and alpha 2-plasmin inhibitor (alpha 2-PI), a factor inhibiting fibrinolysis in blood and gingival tissue of naturally occurring gingivitis rats (ODUS/Odu) and control rats (Res). Blood plasma and supernatants of gingival homogenate from ODUS/Odu and Res were used as samples. And concentrations of alpha 2-PI and AT III were measured by colorimetric assay using commercially available reagents. We found no difference in blood concentrations of alpha 2-PI or AT III between ODUS/Odu and Res groups. However, AT III in the gingiva showed a significantly higher value in the ODUS/Odu group versus that in the controls (p < 0.001). There was a high positive correlation between AT III in gingival tissue and pocket probing depth. Moreover, the fibrinolytic activity in saliva and the gingiva from ODUS/Odu increased with each passing month. Results suggest that both the decrease in blood coagulability and systemic enhancement of fibrinolytic activity due to increased AT III in gingival tissue are associated with the bleeding tendency and inflammatory response in the gingiva of ODUS/Odu.
众所周知，存在多种凝血因子和纤溶因子，并且这些抑制剂也存在于血液和组织中。正常情况下，这些因子相互平衡。我们测定了抗凝血酶III（AT III），它是一种重要的凝血抑制剂，以及α2 - 纤溶酶抑制剂（α2 - PI），它是一种抑制血液和自然发生的牙龈炎大鼠（ODUS/Odu）及对照大鼠（Res）牙龈组织中纤维蛋白溶解的因子。将ODUS/Odu和Res的牙龈匀浆的血浆和上清液用作样本。使用市售试剂通过比色法测定α2 - PI和AT III的浓度。我们发现ODUS/Odu组和Res组之间α2 - PI或AT III的血液浓度没有差异。然而，ODUS/Odu组牙龈中的AT III相对于对照组显示出显著更高的值（p < 0.001）。牙龈组织中的AT III与牙周袋探诊深度之间存在高度正相关。此外，ODUS/Odu组唾液和牙龈中的纤维蛋白溶解活性逐月增加。结果表明，牙龈组织中AT III增加导致的血液凝固性降低和纤维蛋白溶解活性的全身增强均与ODUS/Odu牙龈的出血倾向和炎症反应有关。
