Iga K, Kobayashi H, Mori M
J Periodontol. 1980 Jun;51(6):348-54. doi: 10.1902/jop.1980.51.6.348.
Plaque formation and gingival inflammation were found in ODU plaque-susceptible rats. A study was performed to determine the relationship between the presence of bradykinin in gingival tissue and the inflammatory capacity of rat plaque. Bradykinin content in gingival tissue and the bradykinin inactivating activity of dental plaque of 12-month-old ODU rats were measured. The presence of bradykinin was observed in the gingival tissue of the ODU rat and a bradykinin inactivating factor was found in the rat plaque. The factor was destroyed at 60 degrees C for 30 minutes, at pH 1.5 or by treatment with perchloric acid. The optimum pH of the factor was approximately 8.0. Chelate reagents, such as O-phenanthroline and EDTA . 4Na, strongly inhibited the bradykinin inactivating factor. The protease inhibitor, Trasylol, also inhibited this factor. The SH reagent, L-cysteine, inhibited weakly and Zn++ inhibited less. The investigation suggested that the rat plaque posseses the ability to inactivate bradykinin and plays an important role in modifying the inflammatory response caused by bradykinin in gingival tissue.
在ODU菌斑易感大鼠中发现了斑块形成和牙龈炎症。进行了一项研究以确定牙龈组织中缓激肽的存在与大鼠菌斑炎症能力之间的关系。测量了12月龄ODU大鼠牙龈组织中的缓激肽含量和牙菌斑的缓激肽灭活活性。在ODU大鼠的牙龈组织中观察到缓激肽的存在，并且在大鼠菌斑中发现了一种缓激肽灭活因子。该因子在60℃下30分钟、pH 1.5或用高氯酸处理时被破坏。该因子的最适pH约为8.0。螯合剂，如邻菲罗啉和EDTA·4Na，强烈抑制缓激肽灭活因子。蛋白酶抑制剂抑肽酶也抑制该因子。SH试剂L-半胱氨酸抑制作用较弱，Zn++抑制作用较小。该研究表明，大鼠菌斑具有使缓激肽失活的能力，并在改变缓激肽在牙龈组织中引起的炎症反应中起重要作用。
