Sato M, Mikami O, Kobayashi M, Nakajima Y
Kyushu Research Station, National Institute of Animal Health, 2702 Chuzan, Kagoshima 891-0105, Kagoshima, Japan.
Vet Microbiol. 2000 Jul 3;75(1):1-9. doi: 10.1016/s0378-1135(00)00198-x.
The involvement of apoptosis in the lymphatic organs of piglets infected with classical swine fever (CSF) virus was investigated. Piglets were inoculated with CSF virus and 3, 5, 7 and 10 days post inoculation (DPI), the thymus, spleen and lymph node were examined. In the thymus cortex, macrophages phagocytizing the nuclear remnants or apoptotic bodies increased after 3 DPI. Thymus atrophy due to the loss of the cortex increased markedly during the observation period. Compact and shrunken nuclei indicating apoptosis were observed in the spleen and lymph node. DNA fragmentation was detected in the nuclei of lymphocytes in the thymus, spleen and lymph nodes, as well as at sites of focal necrosis. Using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method, ultrastructural characteristics of apoptosis, i.e. margination of condensed nuclear chromatin, were observed in the lymphatic organs from 2 DPI onward. These results suggest that apoptosis is involved in the pathology of CSF.
研究了细胞凋亡在感染经典猪瘟（CSF）病毒仔猪淋巴器官中的作用。给仔猪接种CSF病毒，在接种后3、5、7和10天（DPI），检查胸腺、脾脏和淋巴结。在胸腺皮质中，3 DPI后吞噬核残余物或凋亡小体的巨噬细胞增多。在观察期内，由于皮质丧失导致的胸腺萎缩明显增加。在脾脏和淋巴结中观察到表明细胞凋亡的致密和萎缩核。在胸腺、脾脏和淋巴结的淋巴细胞核以及局灶性坏死部位检测到DNA片段化。使用末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法，从2 DPI起在淋巴器官中观察到细胞凋亡的超微结构特征，即浓缩核染色质的边缘化。这些结果表明细胞凋亡参与了CSF的病理过程。
