Rerkamnuaychoke B, Chantratita W, Jomsawat U, Thanakitgosate J, Rojanasunan P
Human Genetics Unit, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
J Med Assoc Thai. 2000 Mar;83 Suppl 1:S49-54.
Short tandem repeats (STRs), that represent an important source of highly polymorphic markers in human genome, and mitochondrial DNA (mtDNA) typing, that its sequences were conserved within the same maternal lineage, facilitated by use of the polymerase chain reaction (PCR) provide a powerful tool for forensic identification. We report the analysis of 9 STR loci and mtDNA typing of a muscle biopsied sample with 2 months postmortem by comparison with the genotype of the relative. The DNA profile showed common alleles with that of the relative but only 12 from 20 alleles (60%) were identifiable. Then, we performed mt DNA sequencing of the hypervariable region I (HV I) and obtained 100 per cent homology with that of the relative. In conclusion, personal identification can be performed precisely by the data of DNA profile and mtDNA typing compared to the genotype of the relative.
短串联重复序列（STRs）是人类基因组中高度多态性标记的重要来源，而线粒体DNA（mtDNA）分型（其序列在同一母系谱系中保守）借助聚合酶链反应（PCR）为法医鉴定提供了强大工具。我们报告了对一份死后2个月的肌肉活检样本进行9个STR基因座分析和mtDNA分型，并与亲属的基因型进行比较的情况。DNA图谱显示与亲属有共同等位基因，但20个等位基因中只有12个（60%）可识别。然后，我们对高变区I（HVI）进行了mtDNA测序，结果与亲属的序列100%同源。总之，与亲属的基因型相比，通过DNA图谱和mtDNA分型数据可以精确地进行个人识别。
