Młynarczyk A, Młynarczyk G, Jeljaszewicz J
Zakład Mikrobiologii Lekarskiej Instytutu Biostruktury AM w Warszawie.
Med Dosw Mikrobiol. 1999;51(1-2):9-16.
Localization of genes conferring resistance to MLS, tetracyclines, chloramphenicol, gentamycin and neomycin in 80 MRSA strains isolated from hospital specimens was determined. The obtained results were compared to DNA patterns of the examined strains after digestion with SmaI and separation in pulsed field electrophoresis (PFGE). It was shown that genes of resistance to MLS (ErmI+) in the case of 13 strains were located on chromosome and in the case of 37 strains on plasmids (16 strains had ErmI+ and 21 strains had ErmI-). Genes determining resistance to tetracyclines were localised on chromosome in the case of 39 (23 strains possessed TetK, 11 strains had TetM and 5 strains possessed both TetK and TetM determinants) and in the case of 32 strains on plasmids. Chloramphenicol resistance genes were localised on plasmids in all 30 resistant strains. Genes conferring resistance to gentamycin were present in 31 of the investigated strains on chromosome and in two strains on plasmids. Neomycin resistance genes were plasmid in 34 strains. It was shown that the localization of the resistance genes and the PFGE patterns of the investigated strains were highly correlated.
对从医院标本中分离出的80株耐甲氧西林金黄色葡萄球菌（MRSA）菌株中赋予对MLS、四环素、氯霉素、庆大霉素和新霉素耐药性的基因进行了定位。将所得结果与用SmaI消化并在脉冲场凝胶电泳（PFGE）中分离后的受试菌株的DNA图谱进行比较。结果表明，13株菌株中对MLS（ErmI+）的耐药基因位于染色体上，37株菌株中位于质粒上（16株有ErmI+，21株有ErmI-）。决定对四环素耐药性的基因在39株菌株中位于染色体上（23株具有TetK，11株具有TetM，5株同时具有TetK和TetM决定簇），32株菌株中位于质粒上。所有30株耐药菌株中氯霉素耐药基因均位于质粒上。31株受试菌株中赋予对庆大霉素耐药性的基因位于染色体上，2株位于质粒上。34株菌株中新霉素耐药基因位于质粒上。结果表明，耐药基因的定位与受试菌株的PFGE图谱高度相关。
