Falck P
Acta Biol Med Ger. 1976;35(1):93-101.
Using 125I chemically labelled denatured (d) and native (n) DNA, specifically binding antibodies were demonstrated in the sera of Lupus erythemathodes patients by means of the Farr technique. (NH4)2SO4 was used to separate the immunologically bound 125I-d-DNA. For 125I-n-DNA the use of a secondary antiserum for the precipitation of the primary immune complex is advantageous. The influence of antigen concentration upon the binding rate was studied. Titre determinations can be made with the proposed method.
利用化学标记的125I变性（d）和天然（n）DNA，通过法尔技术在红斑狼疮患者血清中证实了特异性结合抗体。用硫酸铵分离免疫结合的125I-d-DNA。对于125I-n-DNA，使用第二抗血清沉淀一级免疫复合物是有利的。研究了抗原浓度对结合率的影响。可用所提出的方法进行滴度测定。
