Andres G A, Accinni L, Beiser S M, Christian C L, Cinotti G A, Erlanger B F, Hsu K C, Seegal B C
J Clin Invest. 1970 Nov;49(11):2106-18. doi: 10.1172/JCI106428.
Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA. The first group consisted of 13 SLE patients, and the second consisted of 53 patients with non-SLE nephropathies. The data obtained from the two groups of patients were used for comparison, and they showed the following:(a) Fl-Abs to immunoglobulins and complement were bound in the glomeruli of tissues from all patients with active SLE glomerulonephritis characterized by deposits of foreign material in glomerular capillary walls (GCW). The fluorescent pattern was granular, corresponding to the distribution of the glomerular deposits, as seen by electron microscopy. Fl-Abs reactive with thymine and cytosine were bound in the GCW of eight of the nine patients with active SLE glomerulonephritis and showed the same granular distribution. The capacity of these latter Fl-Abs to stain the GCW was removed by absorption with the homologous antigen or denatured DNA.(b) Fl-Abs to immunoglobulins, complement, and pyrimidine bases of DNA did not react with the GCW of two SLE patients without clinical and histologic evidence of glomerulonephritis or with the sclerotic glomeruli of two uremic patients with chronic "burned out" lupus nephritis.(c) The glomeruli of 47 of the 53 patients with other nephropathies bound Fl-Abs to immunoglobulins and complement to some extent, and in 26, the localization appeared as marked as in the patients with active SLE glomerulonephritis. Fl-Abs reactive with thymine and cytosine were bound in the GCW of only one of the renal tissues from the 53 non-SLE patients. In the remaining 52, no binding was seen.(d) The findings are consistent with the hypothesis that antigen-antibody complexes, formed by denatured DNA, specific antibody, and complement, are present in the deposits of foreign material accumulated in the GCW of patients with active SLE glomerulonephritis, and that they may contribute to the pathogenesis of this renal disease.
对两组患者的肾组织进行了研究，使用了荧光素标记（Fl-）的针对免疫球蛋白、补体的抗体，以及用兔制备的针对5-甲基尿苷（T）和胞苷（C）的牛血清白蛋白缀合物的抗体，后两者能与变性DNA特异性反应。第一组由13例系统性红斑狼疮（SLE）患者组成，第二组由53例非SLE肾病患者组成。将两组患者获得的数据进行比较，结果如下：（a）针对免疫球蛋白和补体的Fl-抗体结合于所有活动性SLE肾小球肾炎患者组织的肾小球中，其特征为肾小球毛细血管壁（GCW）中有外来物质沉积。荧光模式呈颗粒状，与电子显微镜下所见的肾小球沉积物分布相对应。与胸腺嘧啶和胞嘧啶反应的Fl-抗体结合于9例活动性SLE肾小球肾炎患者中8例的GCW中，并显示出相同的颗粒状分布。这些后一种Fl-抗体对GCW染色的能力可通过与同源抗原或变性DNA吸收而消除。（b）针对免疫球蛋白、补体和DNA嘧啶碱基的Fl-抗体，不与无肾小球肾炎临床和组织学证据的2例SLE患者的GCW反应，也不与2例患有慢性“终末期”狼疮性肾炎的尿毒症患者的硬化肾小球反应。（c）53例其他肾病患者中的47例肾小球在一定程度上结合了针对免疫球蛋白和补体的Fl-抗体，在26例中，其定位与活动性SLE肾小球肾炎患者一样明显。与胸腺嘧啶和胞嘧啶反应的Fl-抗体仅结合于53例非SLE患者肾组织中的1例GCW中。在其余52例中未观察到结合。（d）这些发现与以下假设一致，即由变性DNA、特异性抗体和补体形成的抗原-抗体复合物存在于活动性SLE肾小球肾炎患者GCW中积累的外来物质沉积物中，并且它们可能促成这种肾脏疾病的发病机制。
