Bonvento G, Cholet N, Seylaz J
Laboratoire de Recherches Cérébrovasculaires, CNRS UPR 646, IFR 6, 10, Avenue de Verdun, 75010, Paris, France.
Neurosci Res. 2000 Jun;37(2):163-6. doi: 10.1016/s0168-0102(00)00109-7.
We examined the effect of type I nitric oxide synthase (neuronal isoform of NOS, nNOS) inhibition on the temporal profile of the cortical blood flow (CoBF) changes induced by a relatively long period (10 min) of whisker stimulation. To address this issue, we used laser-Doppler flowmetry (LDF) to continuously monitor the CoBF in rats anesthetized with alpha-chloralose, in a control condition, and 30 and 60 min following 7-nitroindazole (25 mg/kg, i.p.). Mechanical stimulation of all whiskers for 10 min led to a continuous and sustained CoBF increase with a mean integral response of 4030+/-764%. After 30 and 60 min nNOS inhibition the CoBF response was significantly reduced by 52 and 68%, respectively (P<0. 05) with no evidence of any compensatory mechanism during the whole stimulation period. These data show that regulation of the cerebral blood flow in response to an increased neuronal activity is a dynamic and tonic process in which nNOS plays an essential role.
我们研究了I型一氧化氮合酶（神经元型一氧化氮合酶，nNOS）抑制对相对较长时间（10分钟）的触须刺激所诱导的皮质血流（CoBF）变化的时间特征的影响。为了解决这个问题，我们使用激光多普勒血流仪（LDF）在对照条件下以及腹腔注射7-硝基吲唑（25mg/kg）后30分钟和60分钟，连续监测用α-氯醛糖麻醉的大鼠的CoBF。对所有触须进行10分钟的机械刺激导致CoBF持续增加，平均积分反应为4030±764%。在nNOS抑制30分钟和60分钟后，CoBF反应分别显著降低了52%和68%（P<0.05），在整个刺激期间没有任何代偿机制的证据。这些数据表明，响应神经元活动增加的脑血流调节是一个动态和持续性的过程，其中nNOS起着至关重要的作用。
