Troshina N B, Maksimov I V, Surina O B, Khaĭrullin R M
Department of Biochemistry and Cytochemistry, Ufa Scientific Center, Russian Academy of Sciences, Russia.
Izv Akad Nauk Ser Biol. 2000 May-Jun(3):377-81.
All phases of ontogenesis of Tilletia caries were found in combined suspension and callus culture of the wheat and causative agent of common bunt of wheat. Newly formed spores were observed on calluses of the susceptible species Triticum aestivum and resistant species T. timopheevi within 90 and 120 days after inoculation, respectively, which can be used for their production in the laboratory. On the other hand, susceptibility of the callus culture of the resistant species T. timopheevi suggests different responses of the callus cultures and vegetating plants to the pathogen. The capacity of Tilletia caries of growing on callus culture of T. timopheevi indicates to the possibility of overcoming a resistance barrier of the "timopheevi" type, which would allow a study of this phenomenon for selection purposes.
在小麦与小麦散黑穗病菌的联合悬浮培养和愈伤组织培养中发现了小麦散黑粉菌个体发育的所有阶段。分别在接种后90天和120天,在感病品种普通小麦和抗病品种提莫菲维小麦的愈伤组织上观察到新形成的孢子,这些孢子可用于在实验室中进行生产。另一方面,抗病品种提莫菲维小麦的愈伤组织培养物的易感性表明愈伤组织培养物和活体植物对病原体的反应不同。小麦散黑粉菌在提莫菲维小麦愈伤组织培养物上生长的能力表明有可能克服“提莫菲维”类型的抗性障碍,这将有助于为选择目的研究这一现象。