输出蛋白对SecB的依赖性是一个连续统一体，受信号肽或早期成熟区域特征的影响。
SecB dependence of an exported protein is a continuum influenced by the characteristics of the signal peptide or early mature region.
作者信息
Kim J, Luirink J, Kendall D A
机构信息
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269, USA.
出版信息
J Bacteriol. 2000 Jul;182(14):4108-12. doi: 10.1128/JB.182.14.4108-4112.2000.
Abstract
We have used Escherichia coli alkaline phosphatase to show the interplay among the characteristics of two amino-terminal domains in the preprotein (the signal peptide and the early mature region), the efficiency with which this protein is transported, and its requirement for SecB to accomplish the transport process. The results suggest that although alkaline phosphatase does not normally require SecB for transport, it is inherently able to utilize SecB, and it does so when its ability to interface with the transport machinery is compromised.
摘要
我们利用大肠杆菌碱性磷酸酶来展示前体蛋白中两个氨基末端结构域（信号肽和早期成熟区域）的特征、该蛋白的转运效率以及其完成转运过程对SecB的需求之间的相互作用。结果表明，尽管碱性磷酸酶正常情况下转运不需要SecB，但它本身能够利用SecB，并且当其与转运机制相互作用的能力受损时就会这样做。
相似文献
1
SecB dependence of an exported protein is a continuum influenced by the characteristics of the signal peptide or early mature region.输出蛋白对SecB的依赖性是一个连续统一体，受信号肽或早期成熟区域特征的影响。
J Bacteriol. 2000 Jul;182(14):4108-12. doi: 10.1128/JB.182.14.4108-4112.2000.
2
Identification of a sequence motif that confers SecB dependence on a SecB-independent secretory protein in vivo.体内鉴定赋予SecB依赖性于一种不依赖SecB的分泌蛋白的序列基序。
J Bacteriol. 1998 Mar;180(6):1396-401. doi: 10.1128/JB.180.6.1396-1401.1998.
3
SecA specificity for different signal peptides.SecA对不同信号肽的特异性。
Biochemistry. 2002 Apr 30;41(17):5573-80. doi: 10.1021/bi015798t.
4
[Interaction of SecB and SecA with the N-terminal region of mature alkaline phosphatase on its secretion in Escherichia coli].[SecB和SecA与成熟碱性磷酸酶N端区域在大肠杆菌中分泌过程中的相互作用]
Mol Biol (Mosk). 2003 Jul-Aug;37(4):712-8.
5
High-affinity binding of Escherichia coli SecB to the signal sequence region of a presecretory protein.大肠杆菌SecB与分泌前体蛋白信号序列区域的高亲和力结合。
Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10133-6. doi: 10.1073/pnas.92.22.10133.
6
Preprotein transfer to the Escherichia coli translocase requires the co-operative binding of SecB and the signal sequence to SecA.前体蛋白转移至大肠杆菌转位酶需要SecB和信号序列与SecA协同结合。
Mol Microbiol. 1998 Sep;29(5):1179-90. doi: 10.1046/j.1365-2958.1998.00997.x.
7
SecB-independent export of Escherichia coli ribose-binding protein (RBP): some comparisons with export of maltose-binding protein (MBP) and studies with RBP-MBP hybrid proteins.大肠杆菌核糖结合蛋白（RBP）不依赖SecB的输出：与麦芽糖结合蛋白（MBP）输出的一些比较以及对RBP-MBP杂合蛋白的研究。
J Bacteriol. 1990 Dec;172(12):6875-84. doi: 10.1128/jb.172.12.6875-6884.1990.
8
9
Effect of export-specific cytoplasmic chaperone, protein SecB, on secretion of Escherichia coli alkaline phosphatase.特定于输出的细胞质伴侣蛋白SecB对大肠杆菌碱性磷酸酶分泌的影响。
Biochemistry (Mosc). 2001 Jul;66(7):803-7. doi: 10.1023/a:1010225131673.
10
Synthetic signal peptides specifically recognize SecA and stimulate ATPase activity in the absence of preprotein.合成信号肽在没有前体蛋白的情况下能特异性识别SecA并刺激ATP酶活性。
J Biol Chem. 1998 May 8;273(19):11409-12. doi: 10.1074/jbc.273.19.11409.
引用本文的文献
1
Enhancing Recombinant Protein Yields in the Periplasm by Combining Signal Peptide and Production Rate Screening.通过结合信号肽和生产率筛选提高周质中重组蛋白产量
Front Microbiol. 2019 Jul 23;10:1511. doi: 10.3389/fmicb.2019.01511. eCollection 2019.
2
MatureP: prediction of secreted proteins with exclusive information from their mature regions.MatureP：基于成熟区域特有信息预测分泌蛋白。
Sci Rep. 2017 Jun 12;7(1):3263. doi: 10.1038/s41598-017-03557-4.
3
Sec-dependent protein export and the involvement of the molecular chaperone SecB.依赖Sec的蛋白质输出及分子伴侣SecB的参与。
Cell Stress Chaperones. 2000 Oct;5(4):267-75. doi: 10.1379/1466-1268(2000)005<0267:sdpeat>2.0.co;2.
本文引用的文献
1
Signal peptide determinants of SecA binding and stimulation of ATPase activity.
J Biol Chem. 2000 Apr 7;275(14):10154-9. doi: 10.1074/jbc.275.14.10154.
2
Substrate specificity of the SecB chaperone.SecB伴侣蛋白的底物特异性。
J Biol Chem. 1999 Nov 26;274(48):34219-25. doi: 10.1074/jbc.274.48.34219.
3
Synthetic signal peptides specifically recognize SecA and stimulate ATPase activity in the absence of preprotein.合成信号肽在没有前体蛋白的情况下能特异性识别SecA并刺激ATP酶活性。
J Biol Chem. 1998 May 8;273(19):11409-12. doi: 10.1074/jbc.273.19.11409.
4
The Escherichia coli SRP and SecB targeting pathways converge at the translocon.大肠杆菌的信号识别颗粒（SRP）和SecB靶向途径在转运体处交汇。
EMBO J. 1998 May 1;17(9):2504-12. doi: 10.1093/emboj/17.9.2504.
5
Identification of a sequence motif that confers SecB dependence on a SecB-independent secretory protein in vivo.体内鉴定赋予SecB依赖性于一种不依赖SecB的分泌蛋白的序列基序。
J Bacteriol. 1998 Mar;180(6):1396-401. doi: 10.1128/JB.180.6.1396-1401.1998.
6
Expression of gpsA encoding biosynthetic sn-glycerol 3-phosphate dehydrogenase suppresses both the LB- phenotype of a secB null mutant and the cold-sensitive phenotype of a secG null mutant.编码生物合成型sn-甘油3-磷酸脱氢酶的gpsA的表达既能抑制secB基因缺失突变体的LB-表型，又能抑制secG基因缺失突变体的冷敏感表型。
Mol Microbiol. 1997 Dec;26(5):1013-21. doi: 10.1046/j.1365-2958.1997.6392003.x.
7
Folding of barnase in the presence of the molecular chaperone SecB.在分子伴侣SecB存在的情况下，核酸酶Barnase的折叠。
J Mol Biol. 1997 Nov 28;274(2):268-75. doi: 10.1006/jmbi.1997.1398.
8
Kinetic partitioning. Poising SecB to favor association with a rapidly folding ligand.动力学分配。使SecB保持平衡以利于与快速折叠的配体结合。
J Biol Chem. 1997 Nov 14;272(46):28994-8. doi: 10.1074/jbc.272.46.28994.
9
The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation.在前体蛋白转运起始过程中，分子伴侣SecB从SecA的羧基末端释放出来。
EMBO J. 1997 Oct 15;16(20):6105-13. doi: 10.1093/emboj/16.20.6105.
10
The amino-terminal charge and core region hydrophobicity interdependently contribute to the function of signal sequences.氨基端电荷和核心区域疏水性相互依存地对信号序列的功能起作用。
J Biol Chem. 1996 Aug 30;271(35):21579-82. doi: 10.1074/jbc.271.35.21579.
Zotero 插件