Poole C F
Wayne State University, Department of Chemistry, Rm. 171 Chemistry, Detroit, MI 48202, USA.
J Biochem Biophys Methods. 2000 Jul 5;43(1-3):3-23. doi: 10.1016/s0165-022x(00)00057-9.
This article summarizes recent developments in packed column supercritical fluid chromatography. Silica-based chemically bonded sorbents, similar to those used for HPLC, are widely used with solvent-modified fluids containing additives to suppress undesirable solute-sorbent interactions that lead to poor peak shapes. Composition programming is the most useful approach to gradient elution separations since solvent-modified fluids have low compressibility. Packed column SFC is most useful for the separation of mixtures usually separated by normal-phase HPLC. Compared to normal-phase HPLC it offers faster separations, higher efficiencies, faster column re-equilibration, and a wider range of experimental variables for optimization. Packed column SFC is being increasingly selected for the analytical and preparative separation of racemic mixtures using enantiomer-selective sorbents.
本文总结了填充柱超临界流体色谱的最新进展。类似于用于高效液相色谱（HPLC）的基于硅胶的化学键合吸附剂，广泛用于含有添加剂的溶剂改性流体，以抑制导致峰形不佳的不良溶质-吸附剂相互作用。由于溶剂改性流体具有低压缩性，组成编程是梯度洗脱分离最有用的方法。填充柱超临界流体色谱最适用于分离通常通过正相高效液相色谱分离的混合物。与正相高效液相色谱相比，它具有分离速度更快、效率更高、柱重新平衡更快以及更广泛的用于优化的实验变量范围。使用对映体选择性吸附剂对消旋混合物进行分析和制备分离时，越来越多地选择填充柱超临界流体色谱。
