Ibañez E, Señoráns F J
Instituto de Fermentaciones Industriales, C.S.I.C., Juan de la Cierva, 3, 28006, Madrid, Spain.
J Biochem Biophys Methods. 2000 Jul 5;43(1-3):25-43. doi: 10.1016/s0165-022x(00)00078-6.
The separation of polar compounds by supercritical-fluid chromatography (SFC) is reviewed. New developments in mobile and stationary phase tuning are reviewed for packed and packed capillary SFC. In terms of mobile phase polarity adjustment, new pure and multiple component fluids are presented. The approach of tuning the polarity of the stationary phase as a way of increasing the range of polar compounds analyzed by SFC using pure CO(2) is discussed using either silica-based or new materials as stationary phase. Chiral, liquid crystal and polymer-based stationary phases coated on particles are widely covered in this review as an interesting approach to separate polar compounds avoiding the major drawbacks associated to the use of modifiers in SFC.
本文综述了超临界流体色谱法（SFC）对极性化合物的分离。文中回顾了填充柱和填充毛细管SFC在流动相和固定相调节方面的新进展。在流动相极性调节方面，介绍了新的纯流体和多组分流体。讨论了以调节固定相极性作为一种增加使用纯二氧化碳的SFC分析极性化合物范围的方法，其中固定相采用硅胶基或新型材料。本文广泛涵盖了涂覆在颗粒上的手性、液晶和聚合物基固定相，这是一种分离极性化合物的有趣方法，可避免SFC中使用改性剂带来的主要缺点。
