Yaku K, Morishita F
Analytical Chemistry Department, Product & Technology Development Laboratory, Tanabe Seiyaku Co., Ltd., 16-89, Kashima 3-chome, Yodogawa-ku, 532-8505, Osaka, Japan.
J Biochem Biophys Methods. 2000 Jul 5;43(1-3):59-76. doi: 10.1016/s0165-022x(00)00086-5.
Packed-column supercritical fluid chromatography (pSFC) has been expected to analyze various kinds of compounds. Many researchers have expected a new chromatographic technique that overcomes the limitations of other techniques, HPLC and GC. In pharmaceutical development, chromatography plays an important role in the evaluation of safety and efficacy of a new compound. This article provides an overview of the separation of drugs by pSFC. The effects of the chromatographic parameters were studied for the separation of steroids. In chiral separation, the successful results were shown and compared with HPLC.
填充柱超临界流体色谱法（pSFC）有望用于分析各类化合物。许多研究人员期待一种能克服其他技术（高效液相色谱法和气相色谱法）局限性的新型色谱技术。在药物研发中，色谱法在评估新化合物的安全性和有效性方面发挥着重要作用。本文概述了通过pSFC分离药物的情况。研究了色谱参数对类固醇分离的影响。在手性分离方面，展示了成功的结果并与高效液相色谱法进行了比较。
