Pasetti M F, Pickett T E, Levine M M, Sztein M B
Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, 685 West Baltimore St, Room 480, Baltimore, MD 21201, USA.
Vaccine. 2000 Aug 1;18(28):3208-13. doi: 10.1016/s0264-410x(00)00142-0.
We evaluated the immune responses elicited by attenuated Salmonella enterica serovar Typhi vaccine strain CVD 908-htrA and serovar Typhimurium strain SL3261 alone or as live vectors carrying a plasmid encoding fragment C of tetanus toxin (pTETnir15) in mice immunized intranasally and orogastrically, as well as the in vivo distribution of vaccine organisms following immunization. Higher serologic and proliferative responses against both vector and the foreign antigen were elicited when vaccines were delivered by intranasal route. Whereas both Salmonella strains were detected in the nasal tissue, lungs, and Peyer's patches following intranasal and orogastric immunization, larger numbers of vaccine organisms were recovered from these tissues when the vaccines were delivered intranasally.
我们评估了减毒伤寒沙门氏菌疫苗株CVD 908-htrA和鼠伤寒沙门氏菌菌株SL3261单独或作为携带破伤风毒素片段C编码质粒（pTETnir15）的活载体，经鼻内和经口胃免疫小鼠后引发的免疫反应，以及免疫后疫苗菌在体内的分布。当通过鼻内途径接种疫苗时，针对载体和外源抗原均引发了更高的血清学和增殖反应。经鼻内和经口胃免疫后，在鼻组织、肺和派伊尔结中均检测到了两种沙门氏菌菌株，但当通过鼻内途径接种疫苗时，从这些组织中回收的疫苗菌数量更多。
