牛主动脉中脂蛋白脂肪酶的检测及部分特性分析
Detection and partial characterization of lipoprotein lipase in bovine aorta.
作者信息
Henson L C, Schotz M C
出版信息
Biochim Biophys Acta. 1975 Dec 17;409(3):360-6. doi: 10.1016/0005-2760(75)90031-4.
Extracts of acetone-ether powders of bovine thoracic aorta contain lipase activity which has an alkaline pH maximum (7.8-8.4) and is stimulated 4-10-fold by adding serum or isolated apolipoprotein-glutamate to the assay mixture. Serum activation is completely reversed by isolated apolipoprotein-serine or apolipoprotein-alanine. Lipolysis is strongly inhibited by NaCl (0.5 M) and protamine sulfate (1 mg/ml) and partially inhibited by heparin. Based on these characteristics, the lipase is identified as lipoprotein lipase.
牛胸主动脉丙酮 - 乙醚粉末提取物含有脂肪酶活性，其最适pH值呈碱性（7.8 - 8.4），在测定混合物中添加血清或分离的载脂蛋白 - 谷氨酸可使其活性提高4 - 10倍。血清激活作用可被分离的载脂蛋白 - 丝氨酸或载脂蛋白 - 丙氨酸完全逆转。脂解作用受到NaCl（0.5 M）和硫酸鱼精蛋白（1 mg/ml）的强烈抑制，并受到肝素的部分抑制。基于这些特性，该脂肪酶被鉴定为脂蛋白脂肪酶。
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