博伊丁假丝酵母ADE2基因的克隆与序列分析。

Cloning and sequence analysis of the Candida boidinii ADE2 gene.

作者信息

Komeda T, Suda H, Sakai Y, Kato N, Tamai Y

机构信息

Central Laboratories for Key Technology, Kirin Brewery Co. Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa, 236-0004, Japan.

出版信息

Yeast. 2000 Jul;16(10):953-7. doi: 10.1002/1097-0061(200007)16:10<953::AID-YEA590>3.0.CO;2-D.

DOI:10.1002/1097-0061(200007)16:10<953::AID-YEA590>3.0.CO;2-D

PMID:10870106

Abstract

Candida boidinii ADE2 gene (phosphoribosyl-5-aminoimidazole carboxylase; AIRC, EC 4. 1. 1. 21) has been cloned by homology to the Saccharomyces cerevisiae ADE2 gene. The cloned C. boidinii ADE2 gene complemented the ade2 mutation of S. cerevisiae. Sequence analysis showed a single open reading frame of 1719 nucleotides coding for a polypeptide of 573 residues. Comparison of the deduced amino acid sequence with those of AIRC enzymes from other yeasts showed marked homology among yeast AIRCs.

摘要

博伊丁假丝酵母ADE2基因（磷酸核糖-5-氨基咪唑羧化酶；AIRC，EC 4.1.1.21）已通过与酿酒酵母ADE2基因的同源性克隆得到。克隆的博伊丁假丝酵母ADE2基因弥补了酿酒酵母ade2突变。序列分析显示有一个1719个核苷酸的单一开放阅读框，编码一个由573个残基组成的多肽。将推导的氨基酸序列与其他酵母的AIRC酶的序列进行比较，结果表明酵母AIRC之间存在显著的同源性。