西方许旺酵母中编码磷酸核糖氨基咪唑羧化酶的ADE2基因的序列分析。
Sequence analysis of the ADE2 gene coding for phosphoribosylaminoimidazole carboxylase in Schwanniomyces occidentalis.
作者信息
Gourdon P, Janatova I, Meilhoc E, Klein R D, Costaglioli P, Masson J M
机构信息
Institut National des Sciences Appliquées, CNRS, Toulouse, France.
出版信息
Yeast. 1995 Oct;11(13):1289-93. doi: 10.1002/yea.320111309.
We have determined the nucleotide sequence of a 3.3 kb fragment containing the gene (ADE2) encoding phosphoribosylaminoimidazole carboxylase (AIRC) from the yeast Schwanniomyces occidentalis. Translation of a 1671 bp open reading frame predicts a protein of 557 amino acids which has significant homology to AIRC from Saccharomyces cerevisiae and Schizosaccharomyces pombe. The 5' untranslated region of the S. occidentalis gene contains a sequence corresponding to the consensus binding site of the S. cerevisiae transcription regulatory proteins GCN4, BAS1 and BAS2.
我们已经确定了一个3.3 kb片段的核苷酸序列，该片段包含来自西方施旺酵母（Schwanniomyces occidentalis）的编码磷酸核糖氨基咪唑羧化酶（AIRC）的基因（ADE2）。一个1671 bp开放阅读框的翻译预测出一个由557个氨基酸组成的蛋白质，该蛋白质与酿酒酵母（Saccharomyces cerevisiae）和粟酒裂殖酵母（Schizosaccharomyces pombe）的AIRC具有显著的同源性。西方施旺酵母基因的5'非翻译区包含一个与酿酒酵母转录调节蛋白GCN4、BAS1和BAS2的共有结合位点相对应的序列。
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