Bennett W A, Terrone D A, Rinehart B K, Kassab S, Martin J N, Granger J P
Departments of Obstetrics and Gynecology and Physiology, University of Mississippi Medical Center, Jackson, USA.
Am J Obstet Gynecol. 2000 Jun;182(6):1496-501. doi: 10.1067/mob.2000.106848.
This study was designed to examine the effects of intrauterine endotoxin (lipopolysaccharide) on rat pregnancy.
Pregnant Sprague-Dawley rats (N = 26) were implanted with uterine catheters on day 15 or 16 of a 22-day gestation. Animals were randomly assigned to receive either lipopolysaccharide (25 or 50 microg) or sodium chloride solution (1 mL) on day 17 and then were either sacrificed on day 19 or observed until delivery. Placentas were harvested at the time of death, homogenates were prepared, and prostaglandin F(2)(alpha) metabolite levels were determined by means of radioimmunoassay. Data were analyzed by analysis of variance, Student-Newman-Keuls, and Mann-Whitney tests.
Lipopolysaccharide-treated groups (25 and 50 microg) displayed a shorter interval to delivery (mean +/- SE, 82 +/- 13 and 63 +/- 8 hours, respectively) than control animals (117 +/- 3 hours). Pups of lipopolysaccharide-treated (25 and 50 microg) female animals had lower live birth weights (4.92 +/- 0.01 and 5.12 +/- 0. 24 g, respectively) compared with control animals (6.04 +/- 0.07 g). Placental homogenates from lipopolysaccharide-treated female animals contained higher levels of prostaglandin F(2)(alpha) metabolite (1567 +/- 64 and 1475 +/- 59 pg/mL) than those from sodium chloride solution-infused control animals (804 +/- 68 pg/mL).
Bacterial products induce the preterm delivery of low-birth-weight pups in rats, possibly by increasing local prostaglandin biosynthesis.
本研究旨在探讨宫内内毒素（脂多糖）对大鼠妊娠的影响。
将22天妊娠期第15或16天的怀孕斯普拉格-道利大鼠（N = 26）植入子宫导管。动物在第17天被随机分配接受脂多糖（25或50微克）或氯化钠溶液（1毫升），然后在第19天处死或观察至分娩。死亡时采集胎盘，制备匀浆，并通过放射免疫分析法测定前列腺素F2α代谢物水平。数据采用方差分析、学生-纽曼-库尔斯检验和曼-惠特尼检验进行分析。
脂多糖处理组（25和50微克）的分娩间隔时间（分别为平均±标准误，82±13和63±8小时）比对照组动物（117±3小时）短。与对照组动物（6.04±0.07克）相比，脂多糖处理（25和50微克）雌性动物的幼崽出生体重较低（分别为4.92±0.01和5.12±0.24克）。脂多糖处理雌性动物的胎盘匀浆中前列腺素F2α代谢物水平（1567±64和1475±59皮克/毫升）高于注入氯化钠溶液的对照组动物（804±68皮克/毫升）。
细菌产物可能通过增加局部前列腺素生物合成诱导大鼠早产低体重幼崽。
