Benedetti P, Baldi M I, Mattoccia E, Tocchini-Valentini G P
Istituto di Biologica Cellulare, CNR, Rome, Italy.
EMBO J. 1983;2(8):1303-8. doi: 10.1002/j.1460-2075.1983.tb01585.x.
We have purified to apparent homogeneity a type II DNA topoisomerase from Xenopus laevis oocyte nuclei (germinal vesicles, or GV). The most pure preparations contain a single polypeptide of 175,000 daltons as determined by SDS-gel electrophoresis. The enzyme changes the linking number of DNA circles in steps of two and reversibly knots or catenates DNA rings. No gyrase activity is detectable and ATP is required.
我们已从非洲爪蟾卵母细胞核（生发泡，即GV）中纯化出一种II型DNA拓扑异构酶，达到了明显的均一性。通过SDS-凝胶电泳测定，最纯的制剂含有一条175,000道尔顿的单一多肽。该酶以每次改变两个连环数的步长改变DNA环的连环数，并能可逆地使DNA环打结或形成连环体。未检测到促旋酶活性，且需要ATP。
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