Mizuuchi K, Fisher L M, O'Dea M H, Gellert M
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1847-51. doi: 10.1073/pnas.77.4.1847.
DNA gyrase from Escherichia coli, in the presence of ATP, can both separate catenated DNA circles and unknot knotted DNA. Both these reactions require passage of a DNA segment through a transient double-strand break in DNA. Evidence that transient double-strand breaks are also involved in the supercoiling and relaxing activities of DNA gyrase is derived from experiments showing that the linking number of circular DNA is changed in steps of two. A mechanism is proposed for the action of the enzyme.
来自大肠杆菌的DNA促旋酶在ATP存在的情况下，既能分离连环DNA环，又能解开打结的DNA。这两种反应都需要一段DNA片段穿过DNA中的瞬时双链断裂。有证据表明，瞬时双链断裂也参与了DNA促旋酶的超螺旋和松弛活性，这些证据来自于一些实验，这些实验表明环状DNA的连环数是以两个为单位变化的。本文提出了该酶作用的一种机制。
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