Ramseier H
Exp Cell Biol. 1976;44(1):28-43.
Spleen and lymph node T cells from CBA mice when cultivated in vitro almost immediately started to shed receptors for A alloantigens. In contrast, cultivated T lymphocytes from CBA mice tolerant of A alloantigens for 657 or 692 days began releasing receptors for the formerly tolerated A alloantigen after a delay of 8 h. This delay in receptor shedding coincided with that observed when nontolerant lymphocytes were treated with antisera to T-cell receptors. The results suggested a close similarity of mechanisms and indicated that transplantation tolerance may be maintained by active suppression of otherwise reactive T cells mediated by anti-receptor antibody. Lymphocytes from untreated tolerant mice and those treated with a nonspecific anti-receptor serum showed normal responses, as measured in terms of receptor release, to third-party alloantigens, as did cells from untreated normal mice. Precultivated normal lymphocytes treated with anti-receptor antibody and complement failed to release the appropriate receptor specificity but similarly treated tolerant lymphocytes were almost resistant, presumably because they expressed only few receptors at the time of treatments.
将CBA小鼠的脾脏和淋巴结T细胞进行体外培养时，几乎立刻就开始脱落A同种异体抗原的受体。相比之下，对A同种异体抗原耐受657天或692天的CBA小鼠的培养T淋巴细胞，在延迟8小时后开始释放针对先前耐受的A同种异体抗原的受体。这种受体脱落的延迟与用抗T细胞受体抗血清处理未耐受淋巴细胞时观察到的延迟一致。结果表明机制非常相似，并表明移植耐受性可能通过抗受体抗体介导的对原本有反应的T细胞的主动抑制来维持。从未经处理的耐受小鼠和用非特异性抗受体血清处理的小鼠中获取的淋巴细胞，就受体释放而言，对第三方同种异体抗原的反应正常，未经处理的正常小鼠的细胞也是如此。用抗受体抗体和补体处理的预培养正常淋巴细胞未能释放适当的受体特异性，但同样处理的耐受淋巴细胞几乎具有抗性，推测是因为它们在处理时仅表达很少的受体。
