Wright D A, Huang C P, Chuoke B D
Genetics. 1976 Oct;84(2):319-32. doi: 10.1093/genetics/84.2.319.
A female frog heterozygous at two unlinked loci, specifying electrophoretic forms of mannosephosphate isomerase (MPI) and malate dehydrogenase (MDH) was crossed to male frogs homozygous for different alleles at each locus. In the offspring approximately ten percent proved to be triploid according to nucleolar and chromosome counts of tail tip cells. Most of these triploids had both maternal alleles at the MDH and MPI loci suggesting that the first meiotic division was repressed. Others seemed to represent a repressed second meiotic division and one animal, a pentaploid, could only have resulted from inhibition of both meiotic divisions of the egg. Densitometer tracings of starch gels stained for 6 phosphogluconate and isocitrate dehydrogenases, expected to be heterozygous in a particular cross, demonstrated that the triploids had twice as much maternal as paternal gene product for each locus, similar to patterns found in triploids produced by nuclear transplantation.
一只在两个不连锁基因座上杂合的雌性青蛙，这两个基因座分别决定磷酸甘露糖异构酶（MPI）和苹果酸脱氢酶（MDH）的电泳形式，它与在每个基因座上具有不同等位基因的纯合雄性青蛙杂交。根据尾尖细胞的核仁及染色体计数，后代中约10%被证明是三倍体。这些三倍体中的大多数在MDH和MPI基因座上都有来自母本的两个等位基因，这表明第一次减数分裂受到了抑制。其他的似乎代表了受抑制的第二次减数分裂，还有一只动物是五倍体，它只能是由于卵子的两次减数分裂都受到抑制而产生的。对用6-磷酸葡萄糖酸脱氢酶和异柠檬酸脱氢酶染色的淀粉凝胶进行光密度计扫描，预计在特定杂交中这些酶会是杂合的，结果表明每个基因座的三倍体中母本基因产物是父本的两倍，这与核移植产生的三倍体中发现的模式相似。
