Wright D A, Richards C M, Nace G W
Biochem Genet. 1980 Jun;18(5-6):591-616. doi: 10.1007/BF00484404.
Individuals from natural populations of the leopard frog, Rana pipiens, were analyzed for electrophoretic differences in blood proteins and enzymes from an amputated digit. The proteins examined represent products of 72 loci. Presumptive heterozygotes at multiple loci were selected for experimental crosses. Mendelian inheritance of 18 protein variations were demonstrated in the offspring. Tests for linkage or independent assortment were performed for 75 locus pairs. Three linkage groups were established. Linkage group 1 contains two loci, aconitase-1 (Acon 1) and serum albumin (Alb), with a 19% recombination frequency between them. Linkage group 2 contains four loci, glyoxalase (Gly), acid phosphatase-1 (Ap1), acid phosphatase-2 (AP2), and esterase-5 (Est5). The data show the relationships Gly-21.1%-AP1-0%-AP2-6.3%-Est5, and Gly-25.6%-Est5. Linkage group 3 consists of four closely linked esterase loci. The data, Est1-5.1%-Est6, Est6-1.8%-Est10-1.9%-Est4 and Est6-3.0%-Est4, do not establish a complete order but suggest that Est10 is between Est4 and Est6. These results, with data demonstrating apparent independent assortment of 67 other locus pairs, provide a foundation for establishing the frog genetic map.
对豹蛙（Rana pipiens）自然种群中的个体进行了分析，以研究其断指血液蛋白质和酶的电泳差异。所检测的蛋白质代表了72个基因座的产物。选择多个基因座上的推定杂合子进行实验杂交。在后代中证实了18种蛋白质变异的孟德尔遗传。对75对基因座进行了连锁或独立分配测试。建立了三个连锁群。连锁群1包含两个基因座，乌头酸酶-1（Acon 1）和血清白蛋白（Alb），它们之间的重组频率为19%。连锁群2包含四个基因座，乙二醛酶（Gly）、酸性磷酸酶-1（Ap1）、酸性磷酸酶-2（AP2）和酯酶-5（Est5）。数据显示Gly-21.1%-AP1-0%-AP2-6.3%-Est5以及Gly-25.6%-Est5的关系。连锁群3由四个紧密连锁的酯酶基因座组成。数据Est1-5.1%-Est6、Est6-1.8%-Est10-1.9%-Est4和Est6-3.0%-Est4并未确定完整顺序，但表明Est10在Est4和Est6之间。这些结果以及表明其他67对基因座明显独立分配的数据，为建立青蛙遗传图谱奠定了基础。
