Mukhopadhyay R, Mookerjee A
Int J Radiat Biol Relat Stud Phys Chem Med. 1976 Dec;30(6):543-56. doi: 10.1080/09553007614551401.
The effect of gamma-rays on the binding of proflavine and acridine orange to DNA was investigated by spectrophotometry. The effect of irradiation was observed on the buffered solutions of the free dye and free DNA. A dose of about 35 krad caused a hyperchromicity of 30-40 per cent to the DNA peak at 258 nm, while the same dose introduced a hypochromic effect to the monomer peaks of the dyes by 30 per cent. This implied that gamma-rays have an effect of decreasing the monomer concentration of free-day molecules in solution. From the results, we conclude that more dye is bound to the changed conformation of dye-bound DNA on irradiation. Scratchard-binding isotherms drawn for the unirradiated and irradiated complexes of Pf-DNA showed interesting differences. Similar isotherms could not be obtained for the acridine orange-DNA system.
通过分光光度法研究了γ射线对原黄素和吖啶橙与DNA结合的影响。观察了辐照对游离染料和游离DNA缓冲溶液的影响。约35千拉德的剂量使258nm处DNA峰的增色率达到30%-40%，而相同剂量使染料单体峰产生30%的减色效应。这意味着γ射线具有降低溶液中游离染料分子单体浓度的作用。根据结果，我们得出结论，辐照后更多的染料与染料结合型DNA的构象变化结合。绘制的未辐照和辐照的原黄素-DNA复合物的Scatchard结合等温线显示出有趣的差异。吖啶橙-DNA系统无法获得类似的等温线。
