γ射线辐照对染料-DNH结合的影响。

Effect of gamma irradiation on dye-DNH binding.

作者信息

Mukhopadhyay R, Mookerjee A

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Feb;39(2):143-55. doi: 10.1080/09553008114550161.

Abstract

This paper attempts to analyse the effect of (a) gamma radiation and (b) the presence of histones in the form of nucleohistones (DNH) on the modes of ligand (Proflavine, Pf) binding to DNH. Effects were investigated by spectrophotometry. A certain dose of radiation which causes appreciable DNA denaturation has much less effect on DNH. There is a 'threshold' dose beyond which DNH denaturation is directly proportional to dose as in the case of DNA. The histones in DNH act as a shield and at higher doses get dissociated to expose the double helix to radiations. Ligand binding has a further stabilizing effect against radiation damage to DNH.

摘要

本文试图分析（a）γ辐射和（b）以核组蛋白（DNH）形式存在的组蛋白对配体（原黄素，Pf）与DNH结合模式的影响。通过分光光度法研究其影响。一定剂量的辐射会导致明显的DNA变性，但对DNH的影响要小得多。存在一个“阈值”剂量，超过该剂量后，DNH变性与剂量成正比，如同DNA的情况一样。DNH中的组蛋白起到屏蔽作用，在较高剂量下会解离，使双螺旋暴露于辐射中。配体结合对DNH的辐射损伤具有进一步的稳定作用。