Hokett S D, Cuenin M F, O'Neal R B, Brennan W A, Strong S L, Runner R R, McPherson J C, Van Dyke T E
U.S. Army Periodontic Residency Program, Dental Activity, Fort Gordon, GA 30905, USA.
J Periodontol. 2000 May;71(5):803-9. doi: 10.1902/jop.2000.71.5.803.
Enhanced speed of human gingival fibroblast (HGF) spreading and attachment, as affected by ionic bonding interactions, may facilitate cell orientation and subsequent collagen synthesis to promote early wound healing. The purpose of this study was to determine the in vitro effects of pluronic polyols, a family of widely used surfactants currently used as drug carriers for antibiotic, anti-inflammatory, and anti-neoplastic agents, on the attachment and growth of human gingival fibroblasts (HGF) to dentin and plastic surfaces using established tissue culture techniques.
Plastic culture wells containing Eagle's minimal essential media (EMEM) with 10% fetal calf serum and Pluronic F-68 or F-127 in concentrations from 1.2 x 10(-2) to 1.2 x 10(-10) M were incubated with HGF and run in replicates of ten. Attached cells were quantified by measuring the optical density of methylene blue-stained cells. Additional experiments were conducted using human dentin sections as a substrate and Pluronic F-68 or F-127 at a concentration of 1.2 x 10(-8) M. In these experiments, HGF were stained with acridine orange and quantified per unit area of dentin by fluorescence microscopy.
Attachment and growth of HGF to both plastic and dentin were significantly increased over serum controls by very low concentrations of Pluronic F-68 and F-127 by 30 minutes, with attachment reaching a plateau at 2 hours.
Pluronic polyols, a family of widely used surfactants, in very low dosages may be beneficial in early postsurgical wound healing by facilitating early attachment and enhancing the growth rate of human gingival fibroblasts.
受离子键相互作用影响，人牙龈成纤维细胞（HGF）铺展和附着速度的加快，可能有助于细胞定向及随后的胶原蛋白合成，从而促进早期伤口愈合。本研究的目的是使用既定的组织培养技术，确定普朗尼克多元醇（一类目前用作抗生素、抗炎药和抗肿瘤药载体的广泛使用的表面活性剂）对人牙龈成纤维细胞（HGF）在牙本质和塑料表面附着及生长的体外影响。
将含有伊格尔氏最低限度基本培养基（EMEM）、10%胎牛血清以及浓度为1.2×10⁻²至1.2×10⁻¹⁰ M的普朗尼克F - 68或F - 127的塑料培养孔与HGF一起孵育，并进行十次重复实验。通过测量亚甲蓝染色细胞的光密度对附着细胞进行定量。使用人牙本质切片作为底物并以1.2×10⁻⁸ M的浓度使用普朗尼克F - 68或F - 127进行了额外实验。在这些实验中，HGF用吖啶橙染色，并通过荧光显微镜对每单位面积的牙本质进行定量。
在30分钟时，极低浓度的普朗尼克F - 68和F - 127使HGF在塑料和牙本质上的附着及生长相较于血清对照均显著增加，附着在2小时时达到平台期。
普朗尼克多元醇，一类广泛使用的表面活性剂，极低剂量可能通过促进早期附着和提高人牙龈成纤维细胞的生长速率，对术后早期伤口愈合有益。
