Breault L G, Schuster G S, Billman M A, Hanson B S, Kudryk V L, Pashley D H, Runner R R, McPherson J C
U.S. Army Dental Activity, Ft. Gordon, GA, USA.
J Periodontol. 1995 Jul;66(7):545-51. doi: 10.1902/jop.1995.66.7.545.
To date there has been very little research into the possible effects of endodontic therapy on regeneration of the lost periodontal attachment. The objective of this study was to examine the effects of endodontic medicaments on fibroblast attachment to dentin surfaces free of a smear layer. Pulp chambers of extracted third molars were filled with one of the following medicaments: gutta-percha with Roth's zinc oxide and eugenol-based sealer, warm gutta-percha with sealer, warm gutta-percha without sealer, calcium hydroxide, formocresol, cotton pellet, or left empty. A predetermined dentin surface area was then inoculated with human gingival fibroblasts at a concentration of 2 x 10(4) cells per ml. The cells were allowed to adhere to the dentin surface for either 4 or 24 hours, then cell attachment was quantified using a methyl-tetrazolium assay. The data were analyzed using a Kruskal-Wallis one-way analysis of variance and Dunn's multiple comparison test. It was determined that fibroblast attachment was significantly reduced when exposed to formocresol or warm gutta-percha without sealer at both the 4 and 24 hour interval (P < or = 0.05). This suggests that the use of formocresol or warm gutta-percha without sealer in a root canal may impede periodontal wound healing and regeneration.
迄今为止，关于牙髓治疗对缺失牙周附着再生的可能影响的研究非常少。本研究的目的是检验牙髓药物对成纤维细胞附着于无玷污层牙本质表面的影响。将以下药物之一填充到拔除的第三磨牙的髓腔中：牙胶与含罗斯氧化锌和丁香酚的封闭剂、热牙胶与封闭剂、无封闭剂的热牙胶、氢氧化钙、甲醛甲酚、棉球，或保持空的。然后以每毫升2×10⁴个细胞的浓度将人牙龈成纤维细胞接种到预先确定的牙本质表面积上。使细胞在牙本质表面附着4小时或24小时，然后使用甲基四氮唑测定法对细胞附着进行定量。使用Kruskal-Wallis单向方差分析和邓恩多重比较检验对数据进行分析。结果确定，在4小时和24小时间隔时，当暴露于甲醛甲酚或无封闭剂的热牙胶时成纤维细胞附着显著减少（P≤0.05）。这表明在根管中使用甲醛甲酚或无封闭剂的热牙胶可能会阻碍牙周伤口愈合和再生。
