Langstein J, Becke F M, Söllner L, Krause G, Brockhoff G, Kreutz M, Andreesen R, Schwarz H
Department of Pathology, University of Regensburg, Regensburg, 93042, Germany.
Biochem Biophys Res Commun. 2000 Jun 24;273(1):117-22. doi: 10.1006/bbrc.2000.2889.
CD137 (ILA/4-1BB), a member of the TNF receptor family, regulates activation, survival and proliferation of primary human monocytes. Here we compare the activities of lipopolysaccharide (LPS), a classical and potent monocyte activator to that of CD137. LPS is a more potent activator of monocytes, as evidenced by a stronger induction of the proinflammatory cytokine IL-8. However, CD137 could further increase maximal cytokine induction by LPS, which points to separate signaling pathways for LPS and CD137. Also, expression of myc was only induced by the combination of CD137 and LPS. Expression of macrophage colony-stimulating factor is induced more potently by CD137, but an additive effect is obtained by the combination of CD137 and LPS. Monocyte/macrophage survival and proliferation is only induced by CD137. LPS counteracts both activities of CD137 via activation induced cell death. While LPS has a role in activation of monocytes in innate immunity, the CD137 receptor/ligand system seems to deliver an activating signal to monocyte in acquired immunity.
CD137（ILA/4-1BB）是肿瘤坏死因子受体家族的一员，可调节原代人单核细胞的激活、存活和增殖。在此，我们将脂多糖（LPS）（一种经典且强效的单核细胞激活剂）的活性与CD137的活性进行比较。LPS是更强效的单核细胞激活剂，促炎细胞因子IL-8的诱导作用更强即证明了这一点。然而，CD137可进一步增强LPS诱导细胞因子的最大效应，这表明LPS和CD137的信号通路相互独立。此外，myc的表达仅由CD137和LPS共同诱导产生。巨噬细胞集落刺激因子的表达由CD137诱导作用更强，但CD137与LPS共同作用可产生累加效应。单核细胞/巨噬细胞的存活和增殖仅由CD137诱导产生。LPS通过激活诱导的细胞死亡抵消CD137的这两种活性。虽然LPS在先天免疫中对单核细胞的激活起作用，但CD137受体/配体系统似乎在获得性免疫中向单核细胞传递激活信号。
